rabbit polyclonal anti-pro-sp-c  (Seven Hills Bioreagents)

Journal: Cell reports

Article Title: Dlk1-Mediated Temporal Regulation of Notch Signaling Is Required for Differentiation of Alveolar Type II to Type I Cells during Repair

doi: 10.1016/j.celrep.2019.02.046

Figure Lengend Snippet: KEY RESOURCES TABLE

Article Snippet: Polyclonal rabbit anti-mouse pro-Sp-C (Used at 1:500) , Millipore , RRID AB_91588, Cat# AB3786.

Techniques: Virus, Recombinant, Protease Inhibitor, Red Blood Cell Lysis, SYBR Green Assay, Plasmid Preparation, Reverse Transcription, Bicinchoninic Acid Protein Assay, In Situ, RNA Sequencing, Gene Expression, Real-time Polymerase Chain Reaction, Software, Microscopy, Western Blot

Journal: Physiological Reports

Article Title: Human alveolar epithelial type II cells in primary culture

doi: 10.14814/phy2.12288

Figure Lengend Snippet: Expression of AEII markers and lamellar bodies. (A–D) The isolated AEII cells were cultured for 21 days (A, DIC) and were probed with a rabbit anti-human pro-SPC (SPC) polyclonal antibody (B, red) and a mouse anti-human cytokeratin-8 (CK-8) monoclonal antibody (C, green), followed by incubation with Cy3 Goat anti-Rabbit and FITC Goat anti-Mouse IgG. The SPC and CK-8 were colocalized (D). (E–J) The AEII cells were incubated with LysoTracker® Green DND-26 (E and F) for staining of lamellar bodies, and analyzed under confocal scanning. Green DND-26 staining was negative in human bronchial lung epithelial BEAS-2B cells (G and H) or human lung fibroblasts (I and J).

Article Snippet: The isolated AEII cells were cultured for 21 days and seeded onto glass coverslips at 5 × 10 4 cells/mL in 24-well plates, incubated at 37°C for 12 h, fixed in 4% paraformaldehyde for 10 min, and then were permeabilized using 0.1% Triton X-100 followed by blockade using 2% BSA for 1 h. The cells were probed with rabbit anti-human pro-SP-C polyclonal antibody (Millipore, 1:1000 Temecula, CA), goat anti-human α ENaC polyclonal antibody (Santa Cruz Biotechnology, 1:500, Santa Cruz, CA), mouse anti-human cytokeratin-8 monoclonal antibody (Abcam, 1:100, Cambridge, MA), goat anti-human AQP-5 polyclonal antibody (Santa Cruz Biotechnology, 1:500), for overnight at 4°C.

Techniques: Expressing, Isolation, Cell Culture, Incubation, Staining

Journal: Physiological Reports

Article Title: Human alveolar epithelial type II cells in primary culture

doi: 10.14814/phy2.12288

Figure Lengend Snippet: Expression of SPC and absence of expression of AQP-5 in AEII cell. Cultured AEII cells or Hela cells (5 × 10 4 cells/mL) were seeded for 12 h, fixed in 4% paraformaldehyde, and then were permeabilized using 0.1% Triton X-100 followed by blockade using 2% BSA. The cells were immunostained by using rabbit anti-human pro-SPC polyclonal antibody and goat anti-human AQP-5 polyclonal antibody over night at 4°C. After washing, the cells were incubated with appropriate secondary antibodies for 1 h at 37°C in dark. The coverslips were mounted after washing, and analyzed under a confocal microscope.

Article Snippet: The isolated AEII cells were cultured for 21 days and seeded onto glass coverslips at 5 × 10 4 cells/mL in 24-well plates, incubated at 37°C for 12 h, fixed in 4% paraformaldehyde for 10 min, and then were permeabilized using 0.1% Triton X-100 followed by blockade using 2% BSA for 1 h. The cells were probed with rabbit anti-human pro-SP-C polyclonal antibody (Millipore, 1:1000 Temecula, CA), goat anti-human α ENaC polyclonal antibody (Santa Cruz Biotechnology, 1:500, Santa Cruz, CA), mouse anti-human cytokeratin-8 monoclonal antibody (Abcam, 1:100, Cambridge, MA), goat anti-human AQP-5 polyclonal antibody (Santa Cruz Biotechnology, 1:500), for overnight at 4°C.

Techniques: Expressing, Cell Culture, Incubation, Microscopy